AWWA QTC98241

AWWA QTC98241 Specific Antibodies for Water Testing: the Good the Bad and the IgG1

Conference Proceeding by American Water Works Association, 01/01/1998

Weir, C.; Vesey, G.; Slade, M.; Ferrari, B.; Williams, K.; Veal,D.A.

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A highly antigenic extract of the Cryptosporidium oocyst wall was developed and used to induce a strong IgG response in mice. Following fusion of mouse spleen cells with mouse myeloma cells a hybridoma celline secreting a highly specific IgG1 monoclonal antibody (Cry 104) to the walls of Cryptosporidium oocysts was produced. This antibody has a high specificity for oocysts and does not bind to detritus particles in water and is now in routine use for detecting Cryptosporidium in water.

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