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This paper addresses some of the inadequacies of the current methods and standardsused for evaluating microbial water quality within drinking water systems and source waters. Thegoals were to optimize and implement tangential flow ultrafiltration (TFU) technology for thesimultaneous recovery of low levels of bacteria, viruses, and protozoa from large-volume watersamples; to evaluate quantitative polymerase chain reaction (qPCR) for the detection of selectenteric pathogens; and, to apply these methods to large-volume environmental water samples forthe detection and quantification of microbial contaminants. A TFU method was optimized to simultaneously concentrate and recover each class ofmicroorganism from 100L water samples. Filter type, surfactant addition, and elution steps wereevaluated during TFU optimization. For these evaluations, microbial surrogates including E. coliCN-13, E. faecalis, C. perfringens spores, MS2 and PRD1 bacteriophages, poliovirus and murinenorovirus (MNV-1) were added to either dechlorinated tap water (DTW) or surface water (SW).

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Edition: Vol. - No. Published: 11/01/2009 Number of Pages: 3File Size: 1 file , 830 KB