AWWA WQTC71350

AWWA WQTC71350 Performance-Based Method Systems (PBMS) Validation of a Method 1601 Same-Day Detection of Coliphage in Water

Conference Proceeding by American Water Works Association, 11/01/2009

Clancy, Jennifer L.; Salter, Robert S.; Durbin, Gregory W.; Conklin, Ernestine

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Full Description

Coliphage is one of the indicators that can be used in monitoring under the Ground Water Rule. Unlikebacterial indicators, these viruses have greater survivability and may serve as better predictors of groundwater quality. Simple coliphage methods are needed to indicate fecal and viral contamination of water.Method 1601 is a performance based method where acceptance criteria for method performance weredeveloped in collaborative testing and published as part of the method. Method improvements may bedemonstrated using a Tier 2 study which allows the method validated as an improved U.S. Environmental Protection Agency (USEPA) method. Tier2 validation is done using a prescribed protocol in a multi-lab study where blind samples are provided toeach lab for analysis. The data are analyzed with respect to both recovery and variability within andacross the participating labs. If the results meet the method acceptance criteria, the improved method isconsidered a USEPA validated method for use in water testing. This study describes a 16 hr methodmodification of Method 1601, called Fast Phage, for the detection of coliphage using a 100ml sample witha same day spot step positive prediction using a fluorescent indicator. Includes tables, extended abstract only.

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