AWWA WQTC71531

AWWA WQTC71531 Infectious Cryptosporidium Fractions: Direct Estimates from Rural and Urban Pollution Sources

Conference Proceeding by American Water Works Association, 11/01/2009

Lalancette, Cindy; Di Giovanni, George D.; Genereux, Mylene; Cote, Caroline; Prevost, Michele

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This powerpoint presentation begins by providing a brief overview of the occurrence of Cryptosporidium in both urban and rural environments, and the oocysts percentage of infectivity in riskassessment. Project objectives were to characterize the risk associated with Cryptosporidium infectious oocysts in drinking water sources by: optimizing current cell culture methods by combining total and infectious counts on the same sample; assessing Cryptosporidium infectious fractions levels in rural and urban areas; and, revisiting the risk associated with Cryptosporidium oocysts in drinkingwater via a QMRA approach. Method 3D-CC-IFA, dual direct detection on cell culture with anImmunofluorescent Assay, is presented with the first steps being similar to the U.S. Environmental Protection Agency (USEPA) 1622/23 method. Conclusions are provided for:3D-CC-IFA environmental performance; urban pollution sources; and, rural pollution sources. Includes tables, figures.

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